Hello,

I have a question regarding transcription. I know that RNA-polymerase is moving in the 3'-5' way on DNA and the new RNA is 5'-3' orientation. So if the DNA sequence is 3' ACG TGA 5' RNA sequence is 5' UGC ACU 3'. But what if they say that DNA sequnce is for example 5' CAG TAG 3' ? Then the RNA-polymerase is moving from 3' (back) to 5' (front). So does the enyzime read it like TAG and then CAG, or it goes backwards like GAT GAC?

thank you!

The enzyme will move from 3'-5' and read each base individually so in this case it'll read it as:
GAT GAC.