When you harvest splenocytes from a mouse or so, why is it important when teasing out the cells (via glass slides with frosted edges) not to get cavitation/aerosols? It is going to be centrifuged (isn't that the point of the "wash(s)"?) anyway, so what's the big emphasis of it about?
Cheers,
Phil
Both cavitation and frothing damage and rupture the cells and hence the need to minimise them.
Experts: login to post a reply
Mike Taylor Professionally, I am a computer programmer; I work on dinosaurs in my spare time. I got my Ph.D from the University of Portsmouth in 2009, ...
Dave Warburton I'm Biodiversity Officer with an outer London borough. As such, I provide advice to the council and the public about wildlife matters and legislation, as ...
David Wynick I am Professor of Molecular Medicine at the University of Bristol, and Consultant Physician to the Bristol Royal Infirmary. Over the last 15 years I ...
