Cany anyone explain to how BACs are used to sequence the genome, I've gone over it in my textbook countless times and I feel more confused the more I try to understand it.
My textbook uses words such as microsatellites and clone libraries with no mention as to what they are and why thet are used.
I though sequencing the genome involved using the chain termination process in the automated technique involving PCR and electrophoresis. Apparently BACs are involved before the automated process.
Why can't you just use restriction enzymes or obtaining the mRNA and uding reverse transciptase?